Course "Transmission Electron Microscopy in Life Sciences"
27 November - 1 December 2017, Institute of Molecular Genetics CAS, Prague, Czech Republic
The course "Transmission Electron Microscopy in Life Sciences" is aimed at beginners and intermediate users of transmission electron microscopes in biomedicine. It will devote about equal time to the theory and to practical use of microscopes. The course is limited to 15 participants and during practical sessions the participants will be divided into three groups. The techniques discussed at theoretical sessions will be demonstrated on three transmission electron microscopes of varying complexity - the simplest Morgagni, more complex Philips CM 100 and the most powerful Tecnai T20. After the course, the participants should understand the principles of construction and function of transmission electron microscopes, should be able to align the microscope for optimal performance, to identify and eliminate most common aberrations and alignment artifacts, to understand the principles of image generation in transmission electron microscopy, and to identify optimal ways of image acquisition. The participants will be also given up-to-date information about best ways of sample preparation for transmission electron microscopy, and about recent transmission electron microscopy trends in biomedicine. Participants are encouraged to bring their own samples on 3.05 mm TEM grids. There will be ample opportunity for the participants to discuss their specific problems with the faculty. The course will be taught in English.