Course - Single Molecule Microscopy and Manipulation
October 30. - November 3., 2017, BIOCEV, co-organized by IMCF and Zdeněk Lánský group (IBT)
Using molecular motors and supported lipid bilayers as model systems, and also live cells for some methods, you will carry out state-of-the-art single-molecule detection and imaging using Fluorescence Correlation Spectroscopy (FCS) based methods and Total Internal Reflection Fluorescence (TIRF) microscopy. You will also use Optical Tweezers to manipulate single molecules and to measure forces exerted by single molecular motors.