Course "Biological Specimens in Electron Microscopes"
This practical course is focused on the advanced methods of biological specimen preparation for electron microscopy. The main goal is to make participants familiar with the theoretical background and the latest practical developments in this field. The programme includes state of the art fixation including cryo-methods, cutting of ultrathin sections from embedded or frozen samples, methods of negative staining and immunolabeling, specimen preparation for scanning electron microscopy and methods of specimen preparation for 3D electron microscopy (single particle analysis, electron tomgraphy and serial block face). The results will be evaluated using electron microscopes.
The course is limited to 15 participants. It is intended for Master and PhD students, young researchers, technicians and new users of EM.